《植物生理学报》 2014, 50(11): 1721-1726

油茶良种‘华硕’的组织培养及高效生根

李泽, 谭晓风*, 袁军, 卢锟, 张琳, 林青, 吕佳斌

中南林业科技大学, 经济林培育与保护省部共建教育部重点实验室, 长沙410004

通信作者：谭晓风；E-mail: tanxiaofengcn@126.com；Tel: 0731-85623416

摘　要：

以油茶‘华硕’带芽茎段为外植体, 研究植物生长调节剂、珍珠岩对其快速繁殖及试管苗生根的影响。结果表明: 茎段腋芽萌发的最佳培养基为: MS+2.0 mg•L^-1 6-BA+0.1 mg•L^-1 IAA, 萌发率达88.68%; 最佳继代增殖培养基为: WPM+3.0 mg•L^-1 6-BA+0.01 mg•L^-1 IBA+6.0 mg•L^-1 GA₃, 增殖系数可达11.27; 最佳壮苗伸长培养基为: WPM+0.05 mg•L^-1 IAA+6.0 mg•L^-1 GA₃; 最佳生根培养基为: 1/2MS+1.0 mg•L^-1 IBA+50 g•L^-1珍珠岩, 生根率95.83%。炼苗后移栽到泥炭土、珍珠岩、黄土(1:1:1, V/V/V)混合基质中, 成活率达85%以上。

关键词：油茶; 茎段; 组织培养; 珍珠岩; 快速繁殖

收稿：2014-09-20   修定：2014-10-24

资助：湖南省科技重大专项计划项目(2013FJ1006-3)。

Tissue Culture and Highly Efficient Rooting of Camellia oleifera ‘Huashuo’

LI Ze, TAN Xiao-Feng*, YUAN Jun, LU Kun, ZHANG Lin, LIN Qing, LÜ Jia-Bin

The Key Laboratory of Cultivation and Protection for Non-Wood Forest Trees, Ministry of Education, Central South University of Forestry and Technology, Changsha 410004, China

Corresponding author: TAN Xiao-Feng; E-mail: tanxiaofengcn@126.com; Tel: 0731-85623416

Abstract:

The effects of plant growth regulators and perlite on rooting and rapid propagation were studied using stem with bud of Camellia oleifera ‘Huashuo’ as explants. The results showed that the fittest medium for germination of stem axillary bud was MS+2.0 mg•L^-1 6-BA+0.1 mg•L^-1 IAA, with a 88.68% germination rate of axillary bud; the best medium for shoot multiplication was WPM+3.0 mg•L^-1 6-BA+0.01 mg•L^-1 IBA+6.0 mg•L^-1 GA₃, and the multiplication coefficient reached 11.27; the best medium for seedling elongation culture was WPM+0.05 mg•L^-1 IAA+6.0 mg•L^-1 GA₃; the best rooting medium was 1/2MS+1.0 mg•L^-1 IBA+50 g•L^-1 perlite, with a 95.83% rooting rate. After the culture-bottle seedlings were transplanted into the matrix composed of peat soil, perlite and loess (1:1:1, V/V/V), the survival rate was up to 85%.

Key words: Camellia oleifera; stem; tissue culture; perlite; rapid propagation